光学
偏振器
显微镜
材料科学
旋光法
双折射
显微镜
极化(电化学)
分辨率(逻辑)
摄影术
近场扫描光学显微镜
图像分辨率
数值孔径
光学显微镜
物理
衍射
化学
扫描电子显微镜
波长
物理化学
人工智能
计算机科学
散射
作者
Mahdieh Gholami Mayani,Kim Robert Tekseth,Dag W. Breiby,Jörn Klein,Muhammad Nadeem Akram
出处
期刊:Optics Express
[Optica Publishing Group]
日期:2022-10-06
卷期号:30 (22): 39891-39891
被引量:9
摘要
Polarization-sensitive Fourier-ptychography microscopy (pFPM) allows for high resolution imaging while maintaining a large field of view, and without mechanical movements of optical-setup components. In contrast to ordinary light microscopes, pFPM provides quantitative absorption and phase information, for complex and birefringent specimens, with high resolution across a wide field of view. Using a semi-spherical home-built LED illumination array, a single polarizer, and a 10x /0.28NA objective, we experimentally demonstrate high performance pFPM with a synthesized NA of 1.1. Applying the standard quantitative method, a measured half-pitch resolution of 244 nm is achieved for the 1951 USAF resolution test target. As application examples, the polarimetric properties of a herbaceous flowering plant and the metastatic carcinoma of human liver cells are analyzed and quantitatively imaged.
科研通智能强力驱动
Strongly Powered by AbleSci AI