纳米探针
癌胚抗原
荧光
检出限
生物传感器
注意事项
自体荧光
检测点注意事项
近红外光谱
材料科学
纳米技术
化学
生物医学工程
纳米颗粒
色谱法
光学
医学
病理
癌症
物理
内科学
作者
Zhaorui Song,Yang Suo,Shuang Duan,Shanshan Zhang,Lifu Liu,Botong Chen,Zhen Cheng
标识
DOI:10.1016/j.bios.2023.115063
摘要
Fluorescent lateral flow immunoassay (LFA) is one of the most common analytical platforms for point-of-care testing (POCT), which is capable of facile and early screening of biomarkers. Notably, fluorescent probes play a decisive role in analytical performances of LFA. Herein, we report a novel LFA based on the rare earth doped nanoparticles (RENPs) emitting in the second near-infrared (NIR-II) window for the detection of biomarkers, such as carcinoembryonic antigen (CEA). Benefiting from the dual fluorescent emission at NIR-II window, strong fluorescent penetration, low autofluorescence and excellent photostability of RENPs, this proposed NIR-II LFA displays a good linear relationship ranging from 1 to 320 ng mL-1. The detection limit is as low as 0.37 ng mL-1, which is of 13.5 times lower than the clinical cutoff value. Overall, NIR-II LFA biosensing platform based RENPs not only exhibits high sensitivity, accuracy and specificity, but also have characteristics of rapidity, simplicity and low cost. It holds high potential for early diagnosis of tumor biomarkers in POCT.
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