日本血吸虫
免疫系统
生物
肝星状细胞
免疫学
血吸虫病
血吸虫
纤维化
细胞因子
T细胞
曼氏血吸虫
病理
医学
内分泌学
蠕虫
作者
Jun Ren,Zhuo Yang,Furong He,Lihui Lv,Man Xing,Yingying Guo,Yuchao Zhang,Jiaojiao Liu,Ying Li,Tinghui Bai,Yanan Chen,Guangru Li,Zhiqiang Qin,Dongming Zhou
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:2023-01-01
卷期号:210 (1): 82-95
被引量:3
标识
DOI:10.4049/jimmunol.2200301
摘要
Abstract Schistosomiasis remains an important public health concern. The eggs deposited in livers invoke a Th2-dominant response, which mediates the fibrotic granulomatous response. However, the mechanisms involved in this immunopathological process are still not perfectly clear. Here, we report a single-cell transcriptional landscape of longitudinally collected BALB/c mouse splenocytes at different time points after Schistosoma japonicum infection. We found that exhausted CD4+ T cells were enriched after infection, changing from coproducing multiple cytokines to predominantly producing the Th2 cytokine IL-4. Regulatory B cells had high expression of Fcrl5, Ptpn22, and Lgals1, potentially regulating exhausted CD4+ T cells via direct PD-1–PD-L2 and PD-1–PD-L1 interactions. Within the myeloid compartment, the number of precursor and immature neutrophils sharply increased after infection. Moreover, dendritic cells, macrophages, and basophils showed inhibitory interactions with exhausted CD4+ T cells. Besides, in mouse livers, we found that exhausted CD4+ T cells were distributed around egg granuloma, promoting collagen expression in primary mouse hepatic stellate cells via IL-4 secretion, resulting in liver fibrosis. Our study provides comprehensive characterization of the composition and cellular states of immune cells with disease progression, which will facilitate better understanding of the mechanism underlying liver fibrotic granulomatous response in schistosomiasis.
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