Hyposterolactone A, a 3α‐Hydroxy Steroidal Lactone from the Deep‐Sea‐Derived FungusHypocreasp. ZEN14

Abstract Chemical investigation of the deep‐sea‐derived fungus Hypocrea sp. ZEN14 afforded a new 3α‐hydroxy steroidal lactone, hyposterolactone A ( 1 ) and 25 known secondary metabolites ( 2 – 26 ). The structure of the new compound was established by detailed spectroscopic analysis, electronic circular dichroism (ECD) calculation as well as a J ‐based configuration analysis. Compound 10 showed potent cytotoxicity against Huh7 and Jurkat cells with IC 50 values of 1.4 μM and 6.7 μM, respectively.