CTCF公司
增强子
染色质
发起人
粘蛋白
基因表达调控
生物
分子生物学
转录因子
抄写(语言学)
细胞生物学
基因
遗传学
绝缘体(电)
基因表达
电气工程
工程类
哲学
语言学
作者
Naoki Kubo,Haruhiko Ishii,Xiong Xiong,Simona Bianco,Franz Meitinger,Rong Hu,James D. Hocker,Mattia Conte,David U. Gorkin,Miao Yu,Bin Li,Jesse R. Dixon,Ming Hu,Mario Nicodemi,Huimin Zhao,Bing Ren
标识
DOI:10.1038/s41594-020-00539-5
摘要
The CCCTC-binding factor (CTCF) works together with the cohesin complex to drive the formation of chromatin loops and topologically associating domains, but its role in gene regulation has not been fully defined. Here, we investigated the effects of acute CTCF loss on chromatin architecture and transcriptional programs in mouse embryonic stem cells undergoing differentiation to neural precursor cells. We identified CTCF-dependent enhancer-promoter contacts genome-wide and found that they disproportionately affect genes that are bound by CTCF at the promoter and are dependent on long-distance enhancers. Disruption of promoter-proximal CTCF binding reduced both long-range enhancer-promoter contacts and transcription, which were restored by artificial tethering of CTCF to the promoter. Promoter-proximal CTCF binding is correlated with the transcription of over 2,000 genes across a diverse set of adult tissues. Taken together, the results of our study show that CTCF binding to promoters may promote long-distance enhancer-dependent transcription at specific genes in diverse cell types.
科研通智能强力驱动
Strongly Powered by AbleSci AI