寡核苷酸
工作流程
多路复用
计算机科学
计算生物学
生物信息学
生物
数据库
遗传学
DNA
作者
Michaela Hendling,Ivan Barišić
出处
期刊:Methods in molecular biology
日期:2020-11-13
卷期号:: 19-30
标识
DOI:10.1007/978-1-0716-0822-7_2
摘要
The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.
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