光学相干层析成像
衰减
伤口愈合
疤痕
生物医学工程
反射率
材料科学
成像体模
断层摄影术
光学
病理
医学
核医学
外科
物理
作者
Biswajoy Ghosh,Mousumi Mandal,Pabitra Mitra,Jyotirmoy Chatterjee
标识
DOI:10.1002/jbio.202000357
摘要
Abstract Imaging the structural modifications of underlying tissues is vital to monitor wound healing. Optical coherence tomography (OCT) images high‐resolution sub‐surface information, but suffers a loss of intensity with depth, limiting quantification. Hence correcting the attenuation loss is important. We performed swept source‐OCT of full‐thickness excision wounds for 300 days in mice skin. We used single‐scatter attenuation models to determine and correct the attenuation loss in the images. The phantom studies established the correspondence of corrected‐OCT intensity (reflectivity) with matrix density and hydration. We histologically validated the corrected‐OCT and measured the wound healing rate. We noted two distinct phases of healing—rapid and steady‐state. We also detected two compartments in normal scars using corrected OCT that otherwise were not visible in the OCT scans. The OCT reflectivity in the scar compartments corresponded to distinct cell populations, mechanical properties and composition. OCT reflectivity has potential applications in evaluating the therapeutic efficacy of healing and characterizing scars.
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