The Effects of γ-rays on the Activity of Anti-oxidative Enzymes in Deinococcus Radiodurans

Objective To discuss the relationship between activities of SOD,CAT and radio resistance of Deinococcus radiodurans, Deinococcus radiodurans and Escherichia coli JM109 strain irradiated with different dose of 60 Co γ radiation,and the activities of superoxide dismutase (SOD) and catalase (CAT) of them were compared. Methods The activities of SOD and CAT were determined by Monoamine oxidized method and Ammonium molybdenum acid method respectively. The concentration of protein were determined by Bradford method. Results The activity of SOD and CAT in Deinococcus radiodurans was higher than that in Escherichia coli JM109( P 0.01). After irradiated, the activity of SOD and CAT in Deinococcus radiodurans ascended first, then descended after rea ching the peak. But they almost always descended in JM109 when it was irradiated. After irradiated, the activity of these two enzymes in Deinococcus radiodurans was much higher than that in Escherichia coli JM109( P 0.01). Conclusion Deinococcus radiodurans′ resistance to oxidative stress is higher than Escherichia coli′s.