[Objective] The research aimed to study the effects of soil pH value on physiolovical characteristics of Oryza rufipogon Griff.[Method] Using O.rufipogon as test material,the physiolovical characteristics of O.rufipogon were analyzed through pot experiment under different soil pH value culture.[Result] The chlorophyll content and soluble sugar content were highest,while the proline content and MDA were the lowest at pH=6.8.CAT and POD showed strongest activity at pH=6.8.[Conclusion]O.rufipogon adapt to the pH range form 6.0 to 7.4.