A new pretreatment technology which was used to extract rapeseed oil by combining physical grinding with enzymatic degradation was put forward. A papain was chosen after comparison with other commercial enzymes. The technological condition was optimized in the low water content (1∶0.1~0.4), and the optimal technological condition were as follows: time 2 h, temperature 45 ℃, solid-liquid ratio 1∶0.2, adding amount of enzyme 0.5%. Under this condition the extraction rate was 86.7%.