Objective To study the chemical constituents from Kalimeris indica. Methods Chemical constituents were isolated by column chromatography and semi-prepared HPLC, and the structures were elucidated by spectral data and physical-chemical properties. Results Fifthteen compounds were isolated and respectively identified as friedelin(1), epifriedelanol(2), stimasterol(3), β-sitosterol(4), α-spinasterol(5), erythodiol(6), octadecanoic acid(7), fraxinellone(8), β-daucosterol(9), hexacosanol(10), ergoaterol(11), sphondin(12), dibntyl phthalate(13), oleanic acid(14), and dammerdienly acetate(15). Conclusion Compounds 5, 8—13 are isolated from K. indica for the frist time. Compounds 1, 3, 5, 8, and 12 have inhibitory effects on Cytospora sp. and Rhizoctonia solani.