Determination of lycorine and galantamine contents in Lycoris aurea from different altitudes in Xuefeng Mountain by high performance liquid chromatography

A high performance liquid chromatographic(HPLC)method was developed to determine the contents of lycorine and galantamine in the bulb of Lycoris aurea,and analyze the contents of them in Lycoris aurea populations from different altitudes in Xuefeng Mountain. The chromatographic conditions was as following: 20μL of samples or standards were injected into the Agilent Eclipse XDB-C 18(150 mm × 4. 6 mm,5 μm)column at 25 oC and eluted with mobile phase of water(including 0. 1% phosphoric acid) 65 : methanol 35 at flow rate of 1. 0 mL / min,and the eluents were detected by absorption at 288 nm. The results showed that the contents of lycorine and galantamine of the populations from the different altitudes were different,among which,the contents of them of the population at altitude 678 m were all the highest,about 445. 23μg·g-1and 75. 71μg·g-1respectively. The difference was significant compared with the others. This method was simple,rapid and highly. It was more suitable for cultivating Lycoris aurea in the region of altitude about 678 m in order to extract lycorine and galantamine from the bulb.