Characteristic Analysis of Immunophenotyping Expressions of 282 Cases of Acute Leukemia

Objective To study the characteristic of immunophenotyping expressions of acute leukemia and the diagnostic value of that. Methods 282 cases of acute leukemia were detected by flow cytometry. three color direct immunofluo-rescence staining methods being used. And the antibody integration system was used to classify acute leukemia. Results Acute leukemia were classified into 4 subgruops:monophenotypic acute leukemia,acute leukemia which mainly expressed one lineage-specific antigens,hybrid acute leukemia (HAL) and acute undifferentiated leukemia (AUL). And the percentages of them were 82. 27%,9. 22%,7. 09% and 1. 72% respectively. More than 80% of acute nonlymphocytic leukemias (ANLL) expressed MPO,CD13 and CD33. And more than 90% of acute lymphocytic leukemias (ALL) expressed cCD79a, CD19 or cCD3,CD2. Most of acute leukemias which mainly expressed one lineage-specific antigens were acute B lymphocytic leukemia with expression of myeloid antigen. And most of HAL were acute B lymphocytic leukemia and acute myeloid leukemia. Conclusion cCD3,cCD79a and MPO are lineage-specific antigens,and CD117 is prophase myeloid antigen. And they are important in diagnosis,classification,treatment and prognosis of acute leukemia.