Graphene oxide was synthesized using modified Hummer’s method and assembled in solution and solid surface.The FT-IR、UV-Vis、TGA and SEM were employed to characterize the obtained Graphene oxide and its assemblies.FT-IR showed that the Graphene oxide has oxygen-containing functional groups such as hydroxy,carboxyl,epoxide and carbonyl group.UV-Vis spectra imply the influence of particle size and the pH values on the Graphene oxide dispersion.TGA measurements further confirmed the existence of these functional groups in Graphene oxide.SEM observation showed the micro morphology of the Graphene assembly from solution.