Culture and Identification of Human Umbilical Vein Endothelial Cells

Objective To study the culture method in vitro and identification of human umbilical vein endothelial cells(HUVECs).Methods HUVECs obtained from human umbilical vein by the technique of irrigative digestion,and were cultured with DMEM/F12 mixed culture medium in the carbon dioxide incubator,and were passaged when confluensing.The cultured cells were identified by CD34 immunohisto-chemitry.Results HUVECs could adhere to the plate completely after 24 hours,and confluens a monolayer 4～5 days later.The cultured cells were HUVECs confirmed by CD34 immmunohistochemitry.Conclusion HUVECs can be cultivated and passaged successfully in vitro.