适体
化学
细菌
生物芯片
检出限
原位
纳米颗粒
纳米技术
银纳米粒子
线性范围
致病菌
色谱法
组合化学
分子生物学
材料科学
有机化学
生物
遗传学
作者
Wanli Gao,Bo Li,Ruizhi Yao,Zhiping Li,Xiwen Wang,Xiaolin Dong,Han Qu,Qianxue Li,Nan Li,Hang Chi,Bo Zhou,Zhang Xia
标识
DOI:10.1021/acs.analchem.7b01813
摘要
The characteristic of an ideal bacteria-detection method should have high sensitivity and specificity, be easy to operate, and not have a time-consuming culture process. In this study, we report a new bacteria-detection strategy that can recognize bacteria quickly and directly by surface-enhanced Raman scattering (SERS) with the formation of well-defined bacteria-aptamer@AgNPs. SERS signals generated by bacteria-aptamer@AgNPs exhibited a linear dependence on bacteria (R2 = 0.9671) concentration ranging from 101 to 107 cfu/mL. The detection limit is sensitive down to 1.5 cfu/mL. Meanwhile, the bacteria SERS signal was dramatically enhanced by its specifically recognized aptamer, and the bacteria could be identified directly and visually through the SERS spectrum. This strategy eliminates the puzzling data analysis of previous studies and offers significant advantages over existing approaches, getting a critical step toward the creation of SERS-based biochips for rapid in situ bacteria detection in mixture samples.
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