A356 alloy was refined with a new master alloy of Al-5Ti-B-10Sr. The microstructure and mechanical properties of the refined A356 alloy with different content of master alloy (0, 0.1, 0.3, 0.5 and 0.7 wt.%) were investigated. The results show that the refining effect becomes stronger as the master alloy content increasing from 0.1 to 0.3 wt.%, and then shows slight decrement until 0.7 wt.%. An optimal combination of the tensile strength (193 MPa) and the elongation (17.8%) of as-cast A356 alloy was achieved with 0.3 wt.% of refining master alloy. Compared to the non-modified A356 alloy, the tensile strength and the elongation were improved by 10% and 242%, respectively.