Synthesis of Hybrid Nanoflower-Based Carbonic Anhydrase for Enhanced Biocatalytic Activity and Stability

Carbonic anhydrase (CA) has great potential for CO2 capture; however, the application of CA is still a challenge due to its difficult recovery, low operational stability, and poor reusability. This paper reports the synthesis of hybrid nanoflowers (M–CANFs) by self-assembly of CA and metal phosphates. Mn–CANFs were first discovered in this work although their performance is not excellent, and the performance of M–CANFs formed by different metal phosphates was compared in detail. The results of catalyzing p-nitrophenyl acetate (p-NPA) hydrolysis showed that Ca–CANFs exhibited ca. 286% enhancement of catalytic activity. The stability, reusability, and thermal stability of M–CANFs had enhanced remarkably. The newly formed flowerlike hybrid nanostructures presented multiple flower morphology with great biocatalytic activity and stability enhancement by cycle analysis. The excellent performances are meaningful for biology, catalysis science, and nanomaterials science.