The Optimization of Eluent Chromatography Thin Layer 2-D for The Purification of Isolat Alkaloid of Pulai Having Potention as Anti-Toxoplasma

There was a research having purpose to get pure alkaloid from pulai leaf in a way of chromatography thin layer two dimensions using 2 variants of eluent KLT 2-D. the result of this research is the best profile of chromatography thin layer obtained from eluent chloroform: methanol: ammonia (85: 15: 1) and also from KLT. There was 13 stains where the orange colored alkaloid is on the 8th and 9th stain/ spot. The profile of chromatography thin layer 2-D variant eluent 1 is from phase 1 (stain 1) and phase 2 (stain 2) whereas variant eluent 2 is from phase 1 (3 stains) and phase 2 (1 stain). According to the profile, the best eluent that will be used is variant eluent 2, that is eluent phase 1: chloroform: etil asetat (60:40) and eluent phase 2: chloroform: methanol: ammonia (85: 15:1).