血管生成
后脑
生物
免疫标记
血管
萌芽血管生成
病理
胚胎干细胞
新生血管
体内
神经科学
细胞生物学
中枢神经系统
癌症研究
免疫学
医学
免疫组织化学
内分泌学
基因
遗传学
作者
Gabriela D’Amico,Christiana Ruhrberg
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 3-18
标识
DOI:10.1007/978-1-0716-2059-5_1
摘要
Blood vessel growth is a fundamental process for organ development and wound healing but is also associated with ischemic diseases and cancer. The growth of new blood vessels from preexisting vasculature, termed sprouting angiogenesis, is the predominant mode of blood vessel growth in central nervous system vascularization and pathological vessel growth. Accordingly, studying the molecular and cellular mechanisms of angiogenesis holds the promise to find novel therapeutic targets to stimulate new vessel formation in ischemic tissues or inhibit pathological vessel growth in disease. The embryonic mouse hindbrain provides an excellent model to study sprouting angiogenesis in vivo by histochemical or fluorescent wholemount immunolabeling, thus allowing high-resolution image capture of nascent vasculature and subsequent quantification of relevant angiogenic parameters. This chapter describes how to use the mouse embryonic hindbrain as a model to study physiological angiogenesis, including detailed protocols for hindbrain dissection, wholemount staining, and angiogenic parameters analysis.
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