生物
病毒学
冠状病毒
病毒
猪流行性腹泻病毒
抗病毒药物
冠状病毒科
病毒目
微生物学
腹泻
效价
2019年冠状病毒病(COVID-19)
传染病(医学专业)
医学
疾病
病理
内科学
作者
Rongfeng Tang,Longjun Guo,Qianjin Fan,Liaoyuan Zhang,Yiming Wang,Xin Zhang,Da Shi,Yang Wu,Hongyan Shi,Jianbo Li,Jianfei Chen,Li Feng
标识
DOI:10.1016/j.vetmic.2021.109299
摘要
Porcine deltacoronavirus (PDCoV) is an emerging porcine enteric coronavirus that causes severe diarrhea in piglets and results in serious economic losses. There are no effective vaccines and antiviral drugs to prevent and treat PDCoV infection currently. Griffithsin (GRFT) is a lectin with potent antiviral activity against enveloped viruses because of its ability to specifically bind N-linked high-mannose oligosaccharides. GRFT has been reported to possess antiviral activity against severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and porcine epidemic diarrhea virus (PEDV). Here, we first confirmed the antiviral activity of GRFT against PDCoV in vitro. The infected cells (%) and virus titers were significantly decreased at concentration 1 μg/mL or above of GRFT. Time-course experiments revealed that GRFT inhibits PDCoV infection at the adsorption and penetration step. GRFT binding to PDCoV spike (S) protein on the surface wraps the virus and blocks its entry. The outstanding antiviral potency indicates that GRFT has the potential value as a candidate drug for the prevention and treatment of PDCoV infection.
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