Quantitative HPLC method and alternative green solvents for extraction of [6]-gingerol from ginger.

A simple and validated HPLC method for quantitative analysis of [6]-gingerol in ginger extracts was established. The optimum HPLC conditions involved a TSK-gel ODS-80 Ts column (5 μm, 4.6 ´ 250 mm) eluted with acetonitrile and 1% aqueous acetic acid (48:52, v/v), at 30ºC, with a flow rate of 1 mL/min and a quantitative UV detection at 280 nm. A good selectivity, linearity (R