乙酰化
组蛋白H4
组蛋白
染色质免疫沉淀
分子生物学
染色质
抄写(语言学)
生物
组蛋白脱乙酰基酶
SAP30型
RNA聚合酶Ⅱ
组蛋白H2A
基因
基因表达
发起人
遗传学
哲学
语言学
作者
R. Nicholas Laribee,Michael J. Klemsz
出处
期刊:Biochimica et biophysica acta (N)
[Elsevier]
日期:2005-09-01
卷期号:1730 (3): 226-234
被引量:10
标识
DOI:10.1016/j.bbaexp.2005.08.003
摘要
Although the current paradigm delegates histone deacetylases (HDACs) to the role of transcriptional co-repressors, we recently showed that HDAC activity was necessary for expression of the hematopoietic transcription factor PU.1. Chromatin immunoprecipitation analyses showed that inhibition of HDACs resulted in increased histone H4 acetylation within the promoter and intron 1 regions of the PU.1 locus. In contrast, increases in both H3 and H4 acetylation were seen for introns 2, 3 and 4 on the 3' end of the PU.1 locus. Maximal increases in histone H4 acetylation over the promoter and intron 1 region were seen within 10 min of HDAC inhibition, while the increases seen on the 3' end showed slower kinetics. The increases in H4 acetylation were reversible and decreased levels of acetylation correlated with re-expression of the PU.1 gene. Finally, we show that HDAC activity is required for association of RNA polymerase II with the PU.1 promoter.
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