端粒酶
祖细胞
生物
端粒酶逆转录酶
神经干细胞
端粒
祖细胞
细胞生物学
神经球
人口
细胞分化
干细胞
成体干细胞
遗传学
基因
社会学
人口学
作者
Yun Bai,Qikuan Hu,Xiaoxia Li,Yajun Wang,Chun-Ting Lin,Li Shen,Linsong Li
出处
期刊:Neuroreport
[Ovid Technologies (Wolters Kluwer)]
日期:2004-02-01
卷期号:15 (2): 245-249
被引量:31
标识
DOI:10.1097/00001756-200402090-00006
摘要
It is necessary to expand human neural progenitor cells in vitro to obtain large numbers for research purposes and cell transplantation. A potential obstacle to in vitro expansion, however, is that neural progenitor cells have a limited replication life-span and gradually lose their differentiation potential. We report here that ectopic expression of the catalytic subunit of human telomerase (hTERT) gene in neural progenitor cells could induce telomerase activity, stabilize telomeres and extend their replicative life-spans. The telomerase-immortalized cells (hNPC-TERT) maintained the normal diploid karyotype, expressed the markers of human neural progenitor cells and meanwhile held the differentiation potential in vitro for up to 120 population doublings. This study provides a new approach for obtaining unlimited quantities of normal phenotypic and homogeneous human neural progenitor cells in vitro.
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