纳布芬
色谱法
高效液相色谱法
药代动力学
化学
校准曲线
小猎犬
材料科学
检出限
药理学
医学
生物化学
受体
类阿片
内科学
作者
Carol L. Lake,Cosmo A. DiFazio,Elizabeth N. Duckworth,Jeffrey C. Moscicki,Jeffrey S. Engle,Charles G. Durbin
出处
期刊:Journal of Chromatography B: Biomedical Sciences and Applications
[Elsevier]
日期:1982-12-01
卷期号:233 (1): 410-416
被引量:34
标识
DOI:10.1016/s0378-4347(00)81777-6
摘要
For the determination of nalbuphine and its long acting prodrug, sebacoyl dinalbuphine ester (SDN), in biological samples, a reversed-phase high-performance liquid chromatographic method using dual detectors was established. Ultraviolet and fluorescence detectors were connected in series for determining SDN and nalbuphine, respectively. The two analytes and internal standard were extracted from plasma by alkaline liquid–liquid extraction using n-hexane–isoamyl alcohol (9:1, v/v). The calibration curve for nalbuphine was linear over the range from 10 to 2500 ng/ml, while the range was 25 to 2500 ng/ml for SDN. The within- and between-day precision and accuracy were all within 10% for both nalbuphine and SDN over these concentrations. The method was applied successfully to a pharmacokinetic study of SDN administered at 20 mg/kg to two beagle dogs. Pharmacokinetic analysis revealed that SDN followed a linear one-compartment model with an elimination half-life of 74.7 min. Formation of nalbuphine after intravenous administration of SDN was observed in the first time point sample (5 min). These results indicate that SDN is rapidly metabolized to its active moiety, nalbuphine, in dogs and no other metabolites are detected in plasma.
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