原位
蛋白质组
邻近连接试验
计算生物学
化学
生物化学
生物
细胞生物学
受体
有机化学
作者
Susanne tom Dieck,Lisa Kochen,Cyril Hanus,Maximilian Heumüller,Ina Bartnik,Belquis Nassim-Assir,Katrin Merk,Thorsten Mosler,Sakshi Garg,Stefanie Bunse,David A. Tirrell,Erin M. Schuman
出处
期刊:Nature Methods
[Springer Nature]
日期:2015-03-16
卷期号:12 (5): 411-414
被引量:233
摘要
Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.
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