The N-terminal SH2 domain of Syk is required for (hem)ITAM, but not integrin, signaling in mouse platelets

Key Points Phosphotyrosine-binding capacity of the Syk N-SH2 domain is essential for GPVI and CLEC-2, but not αIIbβ3, activation of mouse platelets. Phosphorylation of Syk on Y519/20, but not of Y346, requires a functional Syk N-terminal SH2 domain.