桃叶珊瑚甙
丙二醛
细胞凋亡
超氧化物歧化酶
活力测定
乳酸脱氢酶
化学
氧化应激
谷胱甘肽过氧化物酶
过氧化氢
过氧化氢酶
谷胱甘肽
药理学
生物化学
抗氧化剂
分子生物学
生物
立体化学
酶
环烯醚萜
糖苷
作者
Huadan Xue,Guizhen Gao,Qiu-Ye Lin,Lei Jin,Yongping Xu
摘要
The present study investigated the neuroprotective effects of aucubin on hydrogen peroxide (H 2 O 2 )‐induced apoptosis in PC12 cells. Exposure of PC12 cells to 0.25 m m H 2 O 2 induced a leakage of lactate dehydrogenase and decreased cell viability, as shown by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide assay. In a dose over 0.1 m m , aucubin increased PC12 cellular viability and markedly attenuated H 2 O 2 ‐induced apoptotic cell death. Quantitation of apoptosis by flow cytometry indicated that aucubin inhibited H 2 O 2 ‐induced apoptosis in PC12 cells. Nuclear damage was alleviated by aucubin, as shown by Hoechst staining. In addition, the levels of malondialdehyde were reduced and the activity of superoxide dismutase, catalase and glutathione peroxidase was augmented in these cells. These results indicated that aucubin inhibited H 2 O 2 ‐induced apoptosis in PC12 cells through regulation of the endogenous oxidant–antioxidant balance. Our results suggest that aucubin is a potential protective agent for the treatment of oxidative‐stress‐induced neurodegenerative disease. Copyright © 2011 John Wiley & Sons, Ltd.
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