骨桥蛋白
小干扰RNA
微泡
肝星状细胞
外体
纤维化
天狼星红
时间1
癌症研究
转化生长因子
细胞生物学
化学
生物
医学
小RNA
免疫学
转染
病理
细胞培养
基因表达
生物化学
遗传学
基因
作者
Min Tang,Cheng Guo,Mucun Sun,Hao Zhou,Xin Peng,Jianli Dai,Qin Ding,Ying Wang,Yang Chen
标识
DOI:10.3389/fphar.2022.882243
摘要
Objective and aims: Osteopontin (OPN), an oxidant stress sensitive cytokine, plays a central role in liver fibrosis. While OPN expression can be reduced by small interfering RNA (siRNA), the challenge to deliver siRNA safely and effectively into liver remains unresolved. Exosomes are promising natural nanocarriers for drug delivery that are able to enter cells with different biological barriers efficiently. In this study, we used exosomes as a delivery vehicle to target OPN in liver fibrosis. Methods: Exosomes selectively home to fibrotic liver according to small animal imaging system. Electroporation technique was used to engineer exosomes to carry siRNA targeting OPN (Exo siRNA−OPN ). Primary hepatic stellate cells (HSCs) were isolated and treated with Exo siRNA−OPN to assess the effect on activated HSCs (aHSCs). Immunofluorescence for α−SMA, an aHSCs marker, and sirius red staining were performed to assess ECM deposition. Finally, plasma OPN from patients with liver fibrosis was identified by ELISA assay. Results: Exosome-mediated siRNA delivery systems show high uptake and low toxicity. Besides, Exo siRNA−OPN suppressed HSCs activation and ECM deposition and more efficiently improved liver function when compared to naked siRNA-OPN. Moreover, Exo siRNA−OPN was assumed inhibiting TGF-β1 signaling activation, along with other fibrotic-related genes based on a GEO datasheet of liver fibrosis samples for correlation analyzes. Exo siRNA−OPN inhibited TGF-β1 signaling by decreasing high-mobility group box-1 (HMGB1). Plasma proteins from chronic HBV-induced fibrosis patients were identified that patients with high OPN expression correlates with more advanced fibrosis progression. Discussion: This study shows that exosome-mediated siRNA-OPN delivery may be an effective option for the treatment of liver fibrosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI