Nuclease‐Mimetic Nanomaterials: From Fundamentals to Bioapplications

With the rapid development of nanozymes and nanomedicine, designing novel nanostructures directly acting on deoxyribonucleic acid (DNA) has great therapeutic potential because DNA is the carrier of genetic information and plays a vital role on life activities of the organism. Specifically, DNA cleavage is an important step in most of these DNA engineering technologies. While nucleases play crucial roles in the cell metabolism by efficient DNA cutting, the practical applications of natural nucleases suffer from some intrinsic shortcomings such as high cost and intolerance to harsh environments. In the past 20 years, great varieties of engineered nanostructures with DNA cleavage (nuclease-mimetic nanomaterials, abbreviated as nuclease mimics) have been developed rapidly and widely used in biomedical fields. In view of the significant progress of nuclease-mimetic nanomaterials, the possible DNA cleavage mechanism mediated by nuclease-mimetic nanomaterials is systematically discussed in this review, and the classification of nuclease-mimetic nanomaterials is illustrated. Their potential biomedical applications, especially in anti-biofilms and cancer treatment, are also comprehensively summarized. Finally, the current opportunities and challenges are discussed to stimulate the research of understanding and development of nuclease-mimetic nanomaterials.