Sulfamethoxazole removal from wastewater via anoxic/oxic moving bed biofilm reactor: Degradation pathways and toxicity assessment

The effects of sulfamethoxazole (SMZ), an antibiotic commonly detected in the water environment, on the performance of a single staged anoxic/oxic moving bed biofilm reactor (A/O MBBR), was investigated. The anoxic zone played a key role in the removal of SMZ with a percentage of contribution accounting for around 85% in the overall removal. Denitrifying heterotrophic microbes present in the anoxic zone showed relatively more resistance to higher SMZ loads. It was found that in extracellular polymeric substances, protein content was increased consistently with the increase in SMZ concentration. Based on the detected biotransformation products, four degradation pathways were proposed and the toxicity was evaluated. Metagenomic analysis revealed that at higher SMZ load the activity of genera, such as Proteobacteria and Actinobacteria was significantly affected. In summary, proper design and operation of staged A/O MBBR can offer a resilient and robust treatment towards SMZ removal from wastewater.