Nanofiber-based Stretchable Electrodes for Oriented Culture and Mechanotransduction Monitoring of Smooth Muscle Cells

纳米纤维 材料科学 电极 佩多:嘘 生物医学工程 静电纺丝 机械转化 纳米技术 血管平滑肌 聚氨酯 表面改性 聚合物 复合材料 平滑肌 化学 细胞生物学 医学 物理化学 内科学 生物
作者
Chen-Xi Bi,Kaili Jin,Jing Yan,Yu Qin,Feng Hong,Wei‐Hua Huang,Yanling Liu
出处
期刊:ACS Sensors [American Chemical Society]
卷期号:8 (8): 3248-3256 被引量:1
标识
DOI:10.1021/acssensors.3c01135
摘要

Vascular smooth muscle cells (SMCs) are circumferentially oriented perpendicular to the blood vessel and maintain the contractile phenotype in physiological conditions. They can sense the mechanical forces of blood vessels expanding and contracting and convert them into biochemical signals to regulate vascular homeostasis. However, the real-time monitoring of mechanically evoked biochemical response while maintaining SMC oriented growth remains an important challenge. Herein, we developed a stretchable electrochemical sensor by electrospinning aligned and elastic polyurethane (PU) nanofibers on the surface of PDMS film and further modification of conductive polymer PEDOT:PSS-LiTFSI-CoPc (PPLC) on the nanofibers (denoted as PPLC/PU/PDMS). The aligned nanofibers on the electrode surface could guide the oriented growth of SMCs and maintain the contractile phenotype, and the modification of PPLC endowed the electrode with good electrochemical sensing performance and stability under mechanical deformation. By culturing cells on the electrode surface, the oriented growth of SMCs and real-time monitoring of stretch-induced H2O2 release were achieved. On this basis, the changes of H2O2 level released by SMCs under the pathology (hypertension) and intervention of natural product resveratrol were quantitatively monitored, which will be helpful to further understand the occurrence and development of vascular-related diseases and the mechanisms of pharmaceutical intervention.
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