催产克雷伯菌
生物
微生物学
克雷伯菌
肺炎克雷伯菌
替加环素
抗菌剂
基因组
亚种
抗生素耐药性
基因
抗生素
遗传学
大肠杆菌
动物
作者
Yang Li,Yun Wu,Dingding Li,Lijun Du,Lu Zhao,Rongxue Wang,Xinfei Chen,Xiaoyun Jia,Ruirui Ma,Tong Wang,Jin Li,Ge Zhang,Xing Wang,Mengting Hu,Xingyu Chen,Xin Wang,Wei Kang,Hongli Sun,Yingchun Xu,Yali Liu
标识
DOI:10.1016/j.jmii.2023.10.014
摘要
The Klebsiella oxytoca complex is an opportunistic pathogen that has been recently identified as an actual complex. However, the characteristics of each species remain largely unknown. We aimed to study the clinical prevalence, antimicrobial profiles, genetic differences, and interaction with the host of each species of this complex.One hundred and three clinical isolates of the K. oxytoca complex were collected from 33 hospitals belonging to 19 areas in China from 2020 to 2021. Species were identified using whole genome sequencing based on average nucleotide identity. Clinical infection characteristics of the species were analyzed. Comparative genomics and pan-genome analyses were performed on these isolates and an augmented dataset, including 622 assemblies from the National Center for Biotechnology Information. In vitro assays evaluating the adhesion ability of human respiratory epithelial cells and survivability against macrophages were performed on randomly selected isolates.Klebsiella michiganensis (46.6%, 48/103) and K. oxytoca (35.92%, 37/103) were the major species of the complex causing human infections. K. michiganensis had a higher genomic diversity and larger pan-genome size than did K. oxytoca. K. michiganensis isolates with blaoxy-5 had a higher resistance rate to various antibiotics, antimicrobial gene carriage rate, adhesion ability to human respiratory epithelial cells, and survival rate against macrophages than isolates of other species.Our study revealed the genetic diversity of K. michiganensis and firstly identified the highly antimicrobial-resistant profile of K. michiganensis carrying blaoxy-5.
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