运动性
Wnt信号通路
连环素
癌变
癌症研究
下调和上调
基因沉默
上皮-间质转换
间充质干细胞
污渍
内科学
医学
细胞生物学
生物
膀胱癌
信号转导
癌症
基因
遗传学
作者
Miaolong Lu,Bolong Liu,Dongyang Li,Zhentao Gao,Wenbiao Li,Xiangfu Zhou,Hailun Zhan
出处
期刊:Life Sciences
[Elsevier BV]
日期:2022-12-06
卷期号:312: 121270-121270
被引量:4
标识
DOI:10.1016/j.lfs.2022.121270
摘要
Although aberrant expression of peroxidasin-like (PXDNL) has been associated with carcinogenesis, its potential role in the Urothelial Carcinoma of the Bladder (UCB) remains unknown. The present study aimed to explore the role of PXDNL in UCB carcinogenesis and its potential clinical value.Based on The Cancer Genome Atlas (TCGA) data, bioinformatics was used to explore the potential clinical value of PXDNL. Wound healing and Transwell invasion assays were employed for the purpose of assessing the cell motility, while the Western Blotting experiments were utilized for investigating the protein expression pattern of PXDNL in UCB and investigating the Epithelial-to-Mesenchymal Transition (EMT) and Wnt/β-catenin pathways for understanding the probable mechanisms involved.PXDNL mRNA was overexpressed in UCB tissues and indicated a poor prognosis. High PXDNL mRNA levels were also associated with advanced clinicopathological features and were regarded as independent prognostic factors for UCB. However, PXDNL showed a weak correlation with immune cell infiltration in UCB. In addition, the findings of the study verified that the existing form of the PXDNL protein was 57-kDa and it was upregulated in the UCB cell lines and tissue samples. Furthermore, silencing PXDNL inhibited, while overexpressing PXDNL promoted EMT and motility of UCB cells in vitro. Mechanistic studies showed that PXDNL activated UCB cell motility via the Wnt/β-catenin pathway.The results reveal a novel molecular target that could be further explored for developing preventive, predictive, and individualized treatment strategies for UCB.
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