UV-Aged Nanoplastics Increase Mercury Toxicity in a Marine Copepod under Multigenerational Exposure: A Carrier Role

under a multigenerational scenario. Aged NPs revealed a better carrier role in Hg bioaccumulation than pristine ones, which was increased by 1.61, 1.52, and 1.54 times in F0, F1, and F2, respectively, probably attributed to increased levels of O-containing functional groups and better adsorption for Hg. Consequently, relative to Hg alone, Hg combined with aged NPs (rather than pristine ones) significantly compromised the copepod's fitness, e.g., the survival rate decreasing by 74.2 and 62.1% in F1 and F2, respectively. This is possibly linked to the most pronounced transcriptomic response under Hg combined with aged NPs, including disturbed cuticle formation, activated antioxidants, and down-regulation of reproductive genes. Overall, our findings emphasize the non-negligible risk of aged NPs as carriers of toxic metals and provide a better understanding about the long-term effects of coexisting NPs and metal pollution on organisms in real marine environments.