CRISPR/Cas9 as precision and high-throughput genetic engineering tools in gastrointestinal cancer research and therapy

清脆的 Cas9 基因组编辑 计算生物学 基因组 生物 癌症 疾病 基因 遗传学 医学 病理
作者
Sayedeh Azimeh Hosseini,Alizamen Salehifard Jouneghani,Mahdi Ghatrehsamani,Hajar Yaghoobi,Fatemeh Elahian,Seyed Abbas Mirzaei
出处
期刊:International Journal of Biological Macromolecules [Elsevier]
卷期号:223: 732-754 被引量:7
标识
DOI:10.1016/j.ijbiomac.2022.11.018
摘要

Gastrointestinal cancer (GI) is one of the most serious and health-threatening diseases worldwide. Many countries have encountered an escalating prevalence of shock. Therefore, there is a pressing need to clarify the molecular pathogenesis of these cancers. The use of high-throughput technologies that allow the precise and simultaneous investigation of thousands of genes, proteins, and metabolites is a critical step in disease diagnosis and cure. Recent innovations have provided easy and reliable methods for genome investigation, including TALENs, ZFNs, and the CRISPR/Cas9 (clustered regularly interspaced palindromic repeats system). Among these, CRISPR/Cas9 has been revolutionary tool in genetic research. Recent years were prosperous years for CRISPR by the discovery of novel Cas enzymes, the Nobel Prize, and the development of critical clinical trials. This technology utilizes comprehensive information on genes associated with tumor development, provides high-throughput libraries for tumor therapy by developing screening platforms, and generates rapid tools for cancer therapy. This review discusses the various applications of CRISPR/Cas9 in genome editing, with a particular focus on genome manipulation, including infection-related genes, RNAi targets, pooled library screening for identification of unknown driver mutations, and molecular targets for gastrointestinal cancer modeling. Finally, it provides an overview of CRISPR/Cas9 clinical trials, as well as the challenges associated with its use.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刘稀发布了新的文献求助10
4秒前
烟花应助SXR采纳,获得10
5秒前
5秒前
灵活性发布了新的文献求助10
5秒前
Y_Jfeng完成签到,获得积分10
7秒前
juejue333完成签到,获得积分10
10秒前
10秒前
cjypdf完成签到,获得积分10
12秒前
smile完成签到,获得积分10
13秒前
Jasper应助科研通管家采纳,获得10
14秒前
SciGPT应助科研通管家采纳,获得10
14秒前
shinysparrow应助科研通管家采纳,获得10
14秒前
脑洞疼应助科研通管家采纳,获得10
14秒前
shinysparrow应助科研通管家采纳,获得10
14秒前
SXR发布了新的文献求助10
14秒前
高血压完成签到,获得积分20
15秒前
cjypdf发布了新的文献求助10
16秒前
帅气天荷完成签到 ,获得积分10
16秒前
瘦瘦的冷之完成签到,获得积分10
21秒前
挥手不再见完成签到 ,获得积分10
21秒前
JamesPei应助取名很难采纳,获得10
21秒前
22秒前
舒服的基德完成签到,获得积分10
24秒前
24秒前
vic发布了新的文献求助10
26秒前
26秒前
27秒前
赘婿应助SXR采纳,获得10
27秒前
完美世界应助飘逸妙芙采纳,获得10
28秒前
29秒前
32秒前
35秒前
AJ发布了新的文献求助30
36秒前
取名很难发布了新的文献求助10
36秒前
vic完成签到,获得积分10
37秒前
程平完成签到,获得积分10
38秒前
39秒前
39秒前
Barry发布了新的文献求助50
41秒前
Jasper应助皓宝牌战斗机采纳,获得30
42秒前
高分求助中
Teaching Social and Emotional Learning in Physical Education 900
Chinese-English Translation Lexicon Version 3.0 500
[Lambert-Eaton syndrome without calcium channel autoantibodies] 440
Plesiosaur extinction cycles; events that mark the beginning, middle and end of the Cretaceous 400
Two-sample Mendelian randomization analysis reveals causal relationships between blood lipids and venous thromboembolism 400
薩提亞模式團體方案對青年情侶輔導效果之研究 400
3X3 Basketball: Everything You Need to Know 310
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2386515
求助须知:如何正确求助?哪些是违规求助? 2092963
关于积分的说明 5266651
捐赠科研通 1819839
什么是DOI,文献DOI怎么找? 907766
版权声明 559181
科研通“疑难数据库(出版商)”最低求助积分说明 484897