Analysis of gene expression of ST13 in tumor cell lines by real-time quantitative PCR

Objective To quantify relatively the gene expression of ST13 in six cell lines by real time quantitative PCR.Methods The total RNA was extracted from cell lines SW620,A549,Bxpc 3,SMMC 7721,RKO and Bcap 37.The RNA was reversely transcripted into cDNA with oligo dT.Then the cDNA was amplified by real time quantitative PCR to quantify the gene expression of ST13 according to an internal control GAPDH.The difference of ST13 gene expression was compared between the cell lines.Results The gene expression of ST13 was as much high as 1% to 20% of GAPDH in the six cell lines respectively.The expression level was divided into three sorts (high,middle and low),showing Bcap 37,RKOSMMC 7721 and Bxpc 3A549,SW620.Conclusion Real time PCR provides a method for monitoring the gene expression of ST13 in cell lines.And it is a useful technique means on gene research.