Effect of NF-κB antisense oligodeoxynucleotides on expression of proinflammatory factors

Objective To study the effect of NF-κB antisense oligodeoxynucleotides (AODN) on expression of NF-κB and proinflammatory factors. Methods The synthesized AODN modified by phosphorothioate at different concentrations were added to the medium of cultured RAW 264.7 cells (preincubation with lipopolysaccharide). Protein expressions of NF-κB p 65 、ICAM、iNOS were detected by SABC immuno-histochemical technique. Results The expressions of NF-κB p 65 、ICAM and iNOS of AODN groups were significantly lower than blank control and MODN groups. From the concentration of 1.0 μM to 5.0 μM , the inhibitory effects increased with the increasing concentration. Conclusion AODN can effectively inhibit the expression of NF-κB protein, thus can depress the expression of proinflammatory factors.