Horseradish peroxidase(HRP) is a peroxidase with heme-containing enzyme for redox-center, which isolated from horseradish roots. In recent years, HRP could be applied for wastewater treatment and food industry, organic synthesis as well as chemiluminescent assays. The structure, immobilization and application of horseradish peroxidase were introduced significantly. The present problems and the future advance in the industry of HRP were reviewed.