Objective: To study anatomical variance and the clinical significance of duodenum-jejunum bend and Treitz ligament. Methods: To summarize the experience that severe dilatation occurred on duodenum in 2 cases after subtotal gastrectomy(Billroth II Method), and observe the anatomical characteristics of duodenum-jejunum bend and Treitz ligament in 151 cases at the same operation. Results: Regardless of importing jejunum anastomosized with the big or small bend of the stomach, there was no duodenum dilatation in 147 cases in which duodenum-jejunum bend located in the ante-centric position of spine and corresponded with the plane of 11, 12 vertebra thoracalis. There were no sequelae in 4 patients having anatomical variance after selecting operation style cautiously according to our clinical experience. Conclusions: For selecting stomach-jejunum inosculate manner correctly, it is essential to observe the anatomic variance of duodenum-jejunum bend, Treitz ligament and other viscera.