Study on the Quality Control Standard of Qizhizhoufei Capsule

Objective:To establish the quality control standard of qizhizhoufei capsule.Method:Radix Astragali,Hirudo,Radix Paeoniae Rubra,Radix et Rhizoma Salviae Miltiorrhizae and Fructus Aurantii Immaturus were identified by TLC.The content of Astragaloside in qizhizhoufei capsule was determined by HPLC.Hypersil ODS-C18(4.6 mm×250 mm,5 μm) was used,with methanol-water(75∶25) as mobile phase,the flow rate was 1.0 mL·min-1,the column temperature was at 40 ℃.Evaporative Light Scattering Detector(ELSD): the temperature of drift tube was 75 ℃,the flow rate of carrier gas was 2.1 L·min-1.Result: The TLC identification methods for the Radix Astragali,Hirudo,Radix Paeoniae Rubra,Radix et Rhizoma Salviae Miltiorrhizae and Fructus Aurantii Immaturus was highly specificity,the negative sample didn't interfere the identification of TLC.The average recoveries(n=6) of astragaloside was 97.97%,RSD was 2.21%.The linear range of astragaloside was 0.28-1.68 μg.Conclusion: The method is simple,repeatable and may be used for the quality control of qizhizhoufei capsule.