Cyclosporin on the Inhibitory Effect of Chemotherapeutic Drugs on Retinoblastoma Cells in Vitro

Objective To evaluate the enhanced effects of different concentrations of cyclosporin on chemotherapeutic drugs (carboplatin and etoposide) for inhibiting SO-Rb50 cells in vitro. Methods The research were divided into 7 groups: G1 (carboplatin group), G2 (carboplatin+0.5 mg/L CSA group), G3(carboplatin+1.0 mg/L CSA group), G4(etoposide group), G5 (etoposide+0.5 mg/L CSA group), G6 (etoposide+1.0 mg/L CSA group), and G7 (different concentrations of CSA alone). The effects of 9 different concentrations ( 0.004 mg/L to 1.0 mg/L double in-creased) of chemotherapeutic drugs (carboplatin and etoposide) and chemotherapeutic drugs mixed with cyclosporin (0.5 mg/L and 1.0 mg/L) on SO-Rb50 cells in vitro were studied respectively. MTT method was used to observe the growth inhibition of SO-Rb50 cells in each group. By comparing with the inhibitory rate of Rb cells and IC50 in each group and the modality changes of Rb cells with converted microscope, we could evaluate the effect of CSA on the chemotherapeutic drugs on Rb cells. Results When the chemotherapeutic drugs (carboplatin and etoposide) mixed with differ-ent concentrations of CSA in vitro, the inhibitory rates of chemotherapeutic drugs on SO-Rb50 cells were higher than that in the chemotherapeutic drugs alone. The IC50 (mg/L) in G1～G6 were : 0.335 ,0.183,0.130,0.113 ,0.099 ,0.077, respectively . When CSA mixed with chemotherapeu-tic drugs and the concentration of CSA increased, the IC50 in each group decreased gradually. There were statistical significant differences in the inhibitory rates and IC50 among G1,G2,G3 and G4,G5,G6 .The inhibitory rate was not related to CSA concentration in G7 group. Under the coverted micro-scope , the modality changes of Rb cells in group with CSA and chemotherapeutic drugs were marked-ly different from that with chemotherapeutic drugs alone. Conclusions CSA alone has no inhibitory ef-fect on Rb cells alone. Different concentrations of CSA can enhanced the inhibitory effects of chemotherapeutic drugs ( carboplatin and etoposide) on Rb cells in vitro. The higher the concentra-tions of CSA, the more significant the inhibitory effects of carboplatin and etoposide on Rb cells .