核酸
生物分子
生物
肽聚糖
聚糖
细菌细胞结构
细胞生物学
小分子
计算生物学
生物化学
细菌
细胞壁
糖蛋白
遗传学
作者
Ozden Kocaoglu,Erin E. Carlson
标识
DOI:10.1038/nchembio.2109
摘要
Biomolecule-specific small-molecule probes, in contrast to genetically encoded tags, can visualize peptidoglycan, lipids, nucleic acids and glycans and have proven useful for imaging of the unique subcellular compartments and environment of bacteria. Fluorescence microscopy is an essential tool for the exploration of cell growth, division, transcription and translation in eukaryotes and prokaryotes alike. Despite the rapid development of techniques to study bacteria, the size of these organisms (1–10 μm) and their robust and largely impenetrable cell envelope present major challenges in imaging experiments. Fusion-based strategies, such as attachment of the protein of interest to a fluorescent protein or epitope tag, are by far the most common means for examining protein localization and expression in prokaryotes. While valuable, the use of genetically encoded tags can result in mislocalization or altered activity of the desired protein, does not provide a readout of the catalytic state of enzymes and cannot enable visualization of many other important cellular components, such as peptidoglycan, lipids, nucleic acids or glycans. Here, we highlight the use of biomolecule-specific small-molecule probes for imaging in bacteria.
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