Intracellular nitrite accumulation: The cause of growth inhibition of Microcystis aeruginosa exposure to high nitrite level

Summary The aim of the present study is to test the role of intracellular nitrite in external nitrite suppressing algal growth. We examined the growth of M icrocystis aeruginosa at different nitrite levels under high nitrate conditions and without nitrate conditions. There were higher intracellular nitrite and lower P m chl a , R d chl a , α chl , maximum cell density and specific growth rate in high nitrate group than nitrate absence group at 5 mg NO 2 − ‐ N L −1 . At 10 and 15 mg NO 2 − ‐ N L −1 , P m chl a , R d chl a , α chl , maximum cell densities and specific growth rates in the high nitrate group became higher than those of the nitrate absence group, while a lower intracellular nitrite in the high nitrate group than nitrate absence group was observed. In addition, the intracellular nitrite and the growth of M . aeruginosa in the high nitrate group did not change from 5 to 10 mg NO 2 − ‐ N L −1 . In the nitrite uptake experiment, with nitrite concentration increasing from 5 to 15 mg NO 2 − ‐ N L −1 , maximum nitrite uptake rate of alga increased, and half‐saturation constant of alga decreased. These results indicate that external nitrite inhibited algal growth through stimulating intracellular nitrite rise, which resulted from overexpression of nitrite transporter.