Diversity of Endogeny Eumycetes in Gynostemma pentaphyllum and Its Correlation with Gypenoside XLIX

Objective: To study the correlation between active components and endogeny eumycetes in Gynostemma pentaphyllum of different types from different habitats. Methods: Endogeny eumycetes from different parts of Gynostemma pentaphyllum were isolated by general isolation methods. Insert method and point planting method were used for identification. The content of gypenoside ⅩLⅠⅩ were determined by HPLC. Results: 125 endogeny eumycetes inhabiting in Gynostemma pentaphyllum were isolated from roots,rhizomes and leaves. By colony morphology and microscopic characteristics,22 genera from 10 families,7 orders,2 classes were identified. Fusarium was the most abundant endogeny eumycetes in Gynostemma pentaphyllum with the account of 22. 4%. Penicillium and Leptosphaeria was12. 8% and 9. 6% respectively. The correlation between Gypenoside ⅩLⅠⅩ and endogeny eumycetes in Gynostemma pentaphyllum was revealed. Conclusion: Endogeny eumycetes are diverse in species and quantity. The endogeny eumycetes species is related to the quality of Gynostemma pentaphyllum.