黄酮醇
化学
抗氧化剂
甲醇
毛蕊糖甙
四氧嘧啶
生物化学
色谱法
胰岛
类黄酮
胰岛素
立体化学
小岛
有机化学
糖尿病
生物
糖苷
医学
内科学
内分泌学
作者
Hyoung‐Geun Kim,Youn Hee Nam,Young Sung Jung,Seon-Min Oh,Trong Nguyen Nguyen,Min-ho Lee,Dae‐Ok Kim,Tong Ho Kang,Dong Hoon Lee,Nam‐In Baek
出处
期刊:Molecules
[MDPI AG]
日期:2021-10-09
卷期号:26 (20): 6098-6098
被引量:13
标识
DOI:10.3390/molecules26206098
摘要
(1) Background: Many flavonoids have been reported to exhibit pharmacological activity; a preparatory study confirmed that Coreopsis lanceolata flowers (CLFs) contained high flavonoid structure content; (2) Methods: CLFs were extracted in aqueous methanol (MeOH:H2O = 4:1) and fractionated into acetic ester (EtOAc), normal butanol (n-BuOH), and H2O fractions. Repeated column chromatographies for two fractions led to the isolation of two aurones and two flavonols; (3) Results: Four flavonoids were identified based on a variety of spectroscopic data analyses to be leptosidin (1), leptosin (2), isoquercetin (3), and astragalin (4), respectively. This is the first report for isolation of 2-4 from CLFs. High-performance liquid chromatography (HPLC) analysis determined the content levels of compounds 1-4 in the MeOH extract to be 2.8 ± 0.3 mg/g (1), 17.9 ± 0.9 mg/g (2), 3.0 ± 0.2 mg/g (3), and 10.9 ± 0.9 mg/g (4), respectively. All isolated compounds showed radical scavenging activities and recovery activities in Caco-2, RAW264.7, PC-12, and HepG2 cells against reactive oxygen species. MeOH extract, EtOAc fraction, and 1-3 suppressed NO formation in LPS-stimulated RAW 264.7 cells and decreased iNOS and COX-2 expression. Furthermore, all compounds recovered the pancreatic islets damaged by alloxan treatment in zebrafish; (4) Conclusions: The outcome proposes 1-4 to serve as components of CLFs in standardizing anti-oxidant, pro-inflammatory inhibition, and potential anti-diabetic agents.
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