细胞毒性T细胞
CD14型
医学
CD16
卵巢癌
肿瘤坏死因子α
流式细胞术
腹水
癌症研究
免疫学
抗原
内科学
生物
癌症
CD3型
体外
CD8型
生物化学
作者
B. Melichar,Cherylyn A. Savary,Rebecca Patenia,Stacie Templin,Karolína Melicharová,Ralph S. Freedman
出处
期刊:International Journal of Gynecological Cancer
[BMJ]
日期:2003-01-01
卷期号:13 (4): 435-443
被引量:4
标识
DOI:10.1136/ijgc-00009577-200307000-00006
摘要
Monocytes/macrophages (MO/MA) represent a major leukocyte population in the peritoneal cavity of patients with epithelial ovarian cancer (EOC). We examined the phenotypic characteristics and antitumor cell activity of ascitic MO in patients with EOC. MO/MA phenotype was compared with MO in peripheral blood by two- and three-color flow cytometry. Cytotoxic/cytostatic effects of different cytokines on cultured EOC cells were measured by initial labeling or uptake inhibition of [methyl- 3 H] thymidine. Malignant ascites had higher proportion of MO/MA with the CD14 bright CD16 + phenotype than peripheral blood. Cell surface antigen expression of activation and differentiation in peripheral blood and ascites, including CD38, CD40, CD64, and CD86, was higher on CD14 bright CD16 − and CD14 bright CD16 + than on CD14 dim CD16 − cells. HLA-DR expression was higher on ascitic MO/MA than peripheral blood MO. Significant cytotoxic/cytostatic activity was elicited by treating ascitic MO/MA with interferon-γ (IFN-γ) and interleukin-2 (IL-2), but not with interleukin-12, paclitaxel, granulocyte-monocyte colony-stimulating factor (GM-CSF), or tumor necrosis factor-alpha (TNF-α). Soluble CD40Lt did not enhance MO/MA cytotoxic activity, and inhibited IFN-γ or IL-2 induced cytoxicity. We conclude that MO/MA from ascites have elevated proportions of CD14 bright CD16 + cells, showing phenotypic features of activation. IFN-γ induces the cytotoxic and cytostatic activity of MO/MA that is inhibited by CD40Lt.
科研通智能强力驱动
Strongly Powered by AbleSci AI