Effect of thiamine pyrophosphate on cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats

焦磷酸硫胺 环磷酰胺 氧化应激 促炎细胞因子 内分泌学 丙二醛 内科学 硫胺素 药理学 化学 医学 卵巢 生物化学 炎症 化疗 辅因子
作者
Mehmet Caner Özer,Sefa İnce,Betül Gündoğdu,Mehmet Aktaş,Kemine Uzel,Cebrail Gürsul,Halis Süleyman,Zeynep Suleyman
出处
期刊:Advances in Clinical and Experimental Medicine [Wroclaw Medical University]
卷期号:31 (2): 129-137 被引量:8
标识
DOI:10.17219/acem/142535
摘要

Background.Cyclophosphamide is a drug used in various types of cancer.It can cause oxidative and inflammatory ovarian damage and infertility.Thiamine pyrophosphate (TPP) to be investigated for its effect on cyclophosphamide-induced ovarian damage and reproductive dysfunction in the present study is the active metabolite of thiamine.It has been shown that TPP protects organs and tissues from oxidative stress and proinflammatory cytokine damage. Objectives.To investigate the effect of TPP against the ovarian damage and reproductive dysfunction caused by cyclophosphamide in rats. Materials and methods.Albino Wistar type female rats were divided into healthy control (HG), cyclophosphamide (CYC) and TPP + cyclophosphamide (TPPC) groups (for each group, n = 12).Thiamine pyrophosphate at a dose of 25 mg/kg was injected intraperitoneally (ip.) in the TPPC group, and 0.9% NaCI solution was injected ip. in the CYC and HG groups.One hour after the injection, 75 mg/kg of cyclophosphamide was administered ip. in the TPPC and CYC groups.This procedure was repeated once a day for 30 days.At the end of this period, 6 rats from each group were euthanized with a high dose of anesthetic (50 mg/kg of sodium thiopental).Biochemical and histopathological examinations were performed on the extracted ovarian tissue.The remaining animals were kept in the laboratory with mature male rats for 2 months for reproduction.Results.Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β).In addition, TPP decreased the severe histopathological damage associated with cyclophosphamide in the ovarian tissue and prevented infertility. Conclusions.Our experimental results have suggested that TPP could be beneficial in the treatment of cyclophosphamide-induced ovarian injury and infertility.
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