肝细胞癌
癌症研究
细胞生长
癌变
小发夹RNA
RNA干扰
医学
生物
基因敲除
癌症
细胞凋亡
内科学
核糖核酸
基因
遗传学
生物化学
作者
Zheping Fang,Bei‐Ge Jiang,Fabiao Zhang,Aidong Wang,Yiming Ji,Yanping Xu,Jicheng Li,Weiping Zhou,Weijie Zhou,Hai-Xiong Han
出处
期刊:Oncotarget
[Impact Journals, LLC]
日期:2014-09-02
卷期号:5 (19): 9256-9268
被引量:8
标识
DOI:10.18632/oncotarget.2389
摘要
The expression of RNA polymerase II subunit 3 (Rpb3) was found frequent up-regulation in Hepatocellular carcinoma (HCC) tumors. Significant associations could also be drawn between increased expressions of Rpb3 and advance HCC staging and shorter disease-free survival of patients. Overexpression of Rpb3 increased HCC cell proliferation, migratory rate and tumor growth in nude mice, whereas suppression of Rpb3 using shRNA inhibited these effects. For mechanism study, we found that Rpb3 bound directly to Snail, downregulated E-cadherin, induced HCC cells epithelial-mesenchymal transition (EMT). In particular, N-terminus of Rpb3 blocked Rpb3 binding to Snail, inhibited Rpb3-high-expression HCC cells proliferation, migration, tumor growth in nude mice, and also inhibited DEN-induced liver tumorigenesis. Furthermore, N-terminus of Rpb3 did not inhibit normal liver cells or Rpb3-low-expression HCC cells proliferation. These findings suggest that N-terminus of Rpb3 selectively inhibits Rpb3-high-expression HCC cells proliferation. N-terminus of Rpb3 may be useful in treating patients diagnosed with Rpb3-high-expression HCC.
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