鞘氨醇
1-磷酸鞘氨醇
鞘氨醇激酶
鞘氨醇激酶1
磷酸酶
基因沉默
细胞生物学
生物
激酶
鞘脂
分子生物学
生物化学
磷酸化
基因
受体
作者
Diana Mechtcheriakova,Alexander Wlachos,Jury Sobanov,Tamara Kopp,Roland Reuschel,Frédéric Bornancin,Richard Cai,Barbara Zemann,Nicole Urtz,Georg Stingl,Gerhard J. Zlabinger,Maximilian Woisetschläger,Thomas Baumruker,Andreas Billich
标识
DOI:10.1016/j.cellsig.2006.09.004
摘要
Sphingosine 1-phosphate (S1P) levels in cells and, consequently, its bioactivity as a signalling molecule are controlled by the action of enzymes responsible for its synthesis and degradation. In the present report, we examined alterations in expression patterns of enzymes involved in S1P-metabolism (sphingosine kinases including their splice variants, sphingosine 1-phosphate phosphatases, and sphingosine 1-phosphate lyase) under certain inflammatory conditions. We found that sphingosine kinase type 1 (SPHK1) mRNA could be triggered in a cell type-specific manner; individual SPHK1 splice variants were induced with similar kinetics. Remarkably, expression and activity of S1P phosphatase 2 (SPP2) was found to be highly upregulated by inflammatory stimuli in a variety of cells (e.g., neutrophils, endothelial cells). Bandshift analysis using oligonucleotides spanning predicted NFκB sites within the SPP2 promoter and silencing of NFκB/RelA via RelA-directed siRNA demonstrated that SPP2 is an NFκB-dependent gene. Silencing of SPP2 expression in endothelial cells, in turn, led to a marked reduction of TNF-α-induced IL-1β mRNA and protein and to a partial reduction of induced IL-8, suggesting a pro-inflammatory role of SPP2. Notably, up-regulation of SPP2 was detected in samples of lesional skin of patients with psoriasis, an inflammatory skin disease. This study provides detailed insights into the regulation of SPP2 gene expression and suggests that SPP2 might be a novel player in pro-inflammatory signalling.
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